TSKgel Boronate-5PW Glass, 10 µm, 1.000 Å, 5.0x50mm

TSKgel Boronate-5PW is based on a 100 nm (1000 Å) pore size polymethacrylate base material bonded with m-aminophenyl boronate. This ligand is capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, glycosolyated proteins, and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. Applications include nucleic acids, catecholamines, nucleosides and nucleotides.

More information