TOYOPEARL AF-rProtein A HC-650F, 45 µm, 1.000 Å, 1L

TOYOPEARL AF-rProtein A HC-650F is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the polymethacrylate base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH. It maintains 80% of its initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F also tolerates exposure to above ambient temperatures.

TOYOPEARL AF-rProtein A HC-650F is available in 45 µm particle size (F-grade) and exhibits dynamic binding capacities of greater than 65 g/L at 5 minutes residence times and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to comparable agarose based resins. It is therefore ideally suited for continuous operation using multiple short columns.

TOYOPEARL AF-rProtein A HC-650F utilizes an alkaline resistant recombinant Protein A variant. This new non-animal derived ligand is bound to the base bead using multipoint attachment. This contributes to both the new resin's alkaline resistance and its low ligand leakages in the range of 5-25 ng ligand/mg IgG.

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