TOYOPEARL AF-rProtein A-650F, 45 µm, 1.000 Å, 100mL

TOYOPEARL AF-rProtein A-650F is an alkaline resistant protein A affinity resin developed for the capture and purification of monoclonal antibodies. TOYOPEARL AF-rProtein A-650F utilizes an alkaline resistant recombinant Protein A variant. This non-animal derived ligand is bound to the base bead using multipoint attachment. This contributes to both the resin's alkaline resistance and its low ligand leakages in the range of 5-25 ng/mg IgG. Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at short residence times.

TOYOPEARL AF-rProtein A-650F exhibits typical dynamic binding capacities of ≥30 g/L for human IgG at 2 minutes residence time and maintains this capacity at increased linear velocities. The resin maintains its capacity well after repeated CIP exposure to 0.1mol/L NaOH and has reasonable tolerance to CIP with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A-650F also tolerates exposure to above ambient temperatures. The resin is available in 45 µm F-grade particle size.  

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