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  • 147,00 CHF - 9.202,00 CHF

Hydrophobic Interaction Chromatography

How does HIC work, click here for the video!

Hydrophobic Interaction Chromatography (HIC) is a powerful tool for the process purification of biomolecules. The technique utilizes the accessible hydrophobic regions located on protein surfaces and their interactions with a weakly hydrophobic stationary phase. Proteins and other molecules with hydrophobic surfaces are attracted to the hydrophobic ligands of HIC resins by employing an aqueous high salt mobile phase. The salt conditions contribute to a lyotropic effect which allows the proteins to bind to a hydrophobic ligand. Proteins are eluted by decreasing the salt concentration. Most therapeutic targets are eluted in a low salt or a no salt buffer. Since HIC separations are done under mild eluting conditions, biological activity is typically retained.

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