Starting in the mid 1970s Reversed Phase Liquid Chromatography (RPC) has become the standard technique to analyze small molecular weight compounds. More recently, RPC has become an accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.
RPC separates molecules based on nonpolar interactions and requires a nonpolar stationary phase and a polar mobile phase. Typically the mobile phase consists of a mixture of water (buffer) and acetonitrile, methanol, THF, or 2-propanol.
